PUBLICATIONS  

197. Scotcher, M. C., and Bennett, G. N. Activity of abrB310 promoter in wild type and spo0A-deficient strains of Clostridium acetobutylicum

196. Sullivan, L., Scotcher, M.C., Bennett, G. N. “Increased biofuel production by metabolic engineering of Clostridium acetobutylicum” in “Microbial Biofuel Conversion: Microbial Contributions to Alternative Fuels” Wall, J., Harwood, C., and Demain, A. (Eds), American Society for Microbiology Press. Chapter in press (2007).

195. Singh, R., Vadlani, P.V., Harrison, M. L., Bennett, G. N., San K.-Y., Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferase AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients. Bioprocess and Biosystems Engineering. In press (2007).

194. Wong, M. S., Wu, S., Causey, T. B., Bennett, G. N., and San, K.-Y. Reduction of acetate production in Escherichia coli for increased recombinant protein production. In press (2007).

193. Wong, M. S., Causey, T. B., Mantzaris, N., Bennett, G. N., and San, K.-Y. Engineering Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Copolymer Composition in E. coli., Biotechnol. Bioeng. In press (2007).

192. Sullivan, L., Paredes, C. J., Papoutsakis, E. T., and Bennett, G. N. Analysis of the clostridial hydrophobic with a conserved tryptophan family (ChW) of proteins in Clostridium acetobutylicum with emphasis on ChW14 and ChW16/1. Enzyme and Microbial Technology. In press (2007).

191. Park, Y. C., San, K. Y., and Bennett, G. N. Characterization of alcohol dehydrogenase 1 and 3 from Neurospora crassa FGSC2489. Appl. Microbiology Biotechnology. In press (2007).

190. Zhu, J., Shalel-Levanon, S., Bennett, G., San, K. Y. The YfiD protein contributes to the pyruvate formate-lyase flux in an Escherichia coli arcA mutant strain. Biotechnol Bioeng. May 1;97(1):138-143 (2007).

189. Kutty R., and Bennett GN. Characterization of a novel ferredoxin with N-terminal extension from Clostridium acetobutylicum ATCC 824. Arch Microbiol. 187, 161-169 (2007).

188. Portle, S, Causey, T. B., Wolf, K., Bennett, G. N., San, K. Y., and Mantzaris, N. Cell population heterogeneity in expression of a gene-switching network with fluorescent markers of different half-lives. J. Biotechnol. 128, 362-375 (2007).

187. Zhu, J., Shalel-Levanon, S., Bennett, G. N., and San, K.-Y. Effect of the global redox sensing/regulation networks on Escherichia coli and the metabolic flux distribution based on C-13 labeling experiments. Metabolic Engineering, 8, 619-627 (2006).

186. Oh, Y., Varmanen, P., Han, X.Y., Bennett, G., Xu, Z., Lu, T., and Palva, A., Lactobacillus plantarum for oral peptide delivery, Oral Microbiology and Immunology, 21, 1-5 (2006).

185. Park, Y. C., Yun, N. R., San, K. Y., and Bennett, G. N. Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene of Candida utilis ATCC 9950. J Ind Microbiol Biotechnol. 33, 1032-1036 (2006).

184. Lin, H., Castro, N.M., Bennett, G. N., and San, K.-Y., Acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation: a potential tool in metabolic engineering, Applied Microbiology and Biotechnology. Aug;71(6):870-4 (2006).

183. Bennett, G.N., and San, K.-Y., Enzyme and co-factor engineering and their applications in the pharmaceutical and fermentation industries, Chapter 8 in Fermentation Microbiology and Biotechnology 2nd edition (E.M.T. El-Mansi, ed), 217-248 (2006).

182. Singh, R., Yang, Y.-T., Lu, B., Bennett, G.N., and San, K.-Y., Expression of the pfl gene and resulting metabolite flux distribution in nuo and ackA-pta E. coli mutant strains. Biotechnol Prog. May-Jun;22(3):898-902 (2006).

181. Sánchez, A.M., Andrews, J., Hussein, I., Bennett, G.N., and San, K.-Y, Effect of overexpression of a soluble pyridine nucleotide transhydrogenase (UdhA) on the production of poly (3-hydroxybutyrate) in Escherichia coli, Biotechnol Prog. Mar-Apr;22(2):420-5 (2006).

180.  Peercy, B.E., Cox, S.J., Shalel-Levanon, S., San, K.-Y., and Bennett, G., A kinetic model of oxygen regulation of cytochrome production in Escherichia coli, J. Theoretical Biol., 242, 547–563, (2006).

179.  Park, Y.C., Choi. J.H., Bennett. G.N., and Seo, J.H. Characterization of d-ribose biosynthesis in Bacillus subtilis JY200 deficient in transketolase gene. J Biotechnol. 121(4):508-16 (2006).

178. Sullivan, L., and Bennett, G.N., Proteome analysis and comparison of Clostridium acetobutylicum ATCC 824 and Spo0A strain variants, J. Ind. Microbiol. Biotechnol., 33(4):298-308 (2006).

177. Dittrich, C.R., San, K.-Y., and Bennett, G.N., Cofactors in metabolic engineering of ester production, Proceedings of the American Chemical Society Symposium on Genetic Engineering in Flavor Chemistry: Applications and Potentials, ACS Press, in press.

176. Kutty, R., Rudolph, F.B., and Bennett, G.N., Transformation of 2,4,6-trinitrotoluene by Fe-hydrogenase from Clostridium acetobutylicum ATCC824: Relevance to mechanism of catalysis, in Proceedings Remediation of Chlorinated and Recalcitrant Compounds: The Fourth International Conference, May 24-27, 2004, Monterey, California, Battelle Press, Columbus, OH, (2005).

175. Kutty, R., and Bennett, G.N., Biochemical characterization of trinitrotoluene transforming oxygen-insensitive nitroreductases from Clostridium acetobutylicum ATCC 824, Arch. Microbiol., Nov;184(3):158-67, (2005).

174. Vadali, R.V., Fu. Y., Bennett, G.N., and San, K.Y., Enhanced lycopene productivity by manipulation of carbon flow to isopentenyl diphosphate in Escherichia coli, Biotechnol, Prog., 21, 1558-1561 (2005).

173. Lin, H., Bennett, G.N., and San, K.-Y., Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: A study of the modified metabolic network based on metabolite profile, enzyme activity, and gene expression profile, Metabolic Engineering., 7(5-6):337-52 (2005).

172. Shalel-Levanon, S., San, K.-Y., and Bennett, G.N., Effect of oxygen, and Arca and FNR regulators on the expression of genes related to the electron transfer chain and the TCA cycle in Escherichia coli, Metabolic Engineering, 7, 364-374 (2005).

171. Shalel-Levanon, S., San, K.-Y., and Bennett, G.N., Effect of ArcA and FNR on the expression of genes related to the oxygen regulation and the glycolysis pathway in Escherichia coli under microaerobic growth conditions, Biotechnol. Bioeng., 92, 147-159 (2005).

170. Sanchez AM, Bennett GN, San KY. Batch culture characterization and metabolic flux analysis of succinate-producing Escherichia coli strains. Metabolic Engineering. 8(3):209-26 (2006).

169. Cox, S.J., Shelel Levanon, S., Sanchez, A., Lin, H., Peercy, B., Bennett, G.N., and San, K.-Y., Development of a metabolic network design and optimization framework incorporating implementation constraints: A succinate production case study, Metabolic Engineering, Jan;8(1):46-57 (2006).

168. Horton, C.E., and Bennett, G.N., Ester production in E. coli and C. acetobutylicum, Enzyme and Microbial Technology, 38, 937-943 (2006).

167. Kutty, R., and Bennett, G.N., Studies on inhibition of transformation of 2,4,6-trinitrotoluene catalyzed by Fe-only hydrogenase from Clostridium acetobutylicum, J. Ind. Microbiol. Biotechnol. 33(5):368-76 (2006).

166. Yun, N.-R., San, K.-Y., and Bennett, G.N., Enhancement of lactate and succinate formation in adhE or pta-ackA in NADH dehydrogenase-deficient Escherichia coli, Journal of Applied Microbiology, 99,1404-1412 (2005).

165. Sánchez, A.M., Bennett, G.N., and San, K.-Y., Effect of different levels of NADH availability on metabolic fluxes of Escherichia coli chemostat cultures in defined medium, J. Biotechnol., 117, 395-405 (2005).

164. Sanchez, A.M., Bennett, G.N., and San, K.-Y., Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity, Metabolic Engineering, 7, 229-239 (2005).

163. Scotcher, M.C., Rudolph, F.B., and Bennett, G.N., The expression of abrB310 and sinR, and the effects of AbrB310 on the transition from acidogenesis to solventogenesis in Clostridium acetobutylicum ATCC824, Applied and Environmental Microbiology, 71, 1987-1995 (2005).

162. Scotcher, M.C., and Bennett, G.N., SpoIIE regulates sporulation but does not directly affect solventogenesis in Clostridium acetobutylicum ATCC824, J. Bacteriology, 187, 1930-1936 (2005).

161. Lin, H., Bennett, G.N., and San, K.-Y., Fed-batch culture of a metabolically engineered Escherichia coli strain designed for high-level succinate production and yield under aerobic conditions, Biotechnol. Bioeng., 90, 775-779 (2005).

160. Lin, H., Bennett, G.N., and San, K.-Y., Effect of carbon sources differing in oxidation state and transport route on succinate production in metabolically engineered Escherichia coli, J. Ind. Microbiol. Biotechnol., 32, 87-93 (2005).

159. Lin, H., Bennett, GN., and San, K.-Y., Effect of Sorghum vulgare phosphoenolpyruvate carboxylase and Lactococcus lactis pyruvate carboxylase coexpression on succinate production in mutant strains of Escherichia coli, Appl. Microbiol. Biotechnol., 67, 515-523 (2005).

158. Dittrich, C.R., Vadali, R.V., Bennett, G.N., and San, K.-Y., Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB pathways for the synthesis of isoamyl acetate, Biotechnol. Prog., 21, 627-631 (2005).

157. Dittrich, C.R., Bennett, G.N., and San, K.-Y., Characterization of the acetate-producing pathways in Escherichia coli, Biotechnol. Prog., 21, 1062-1067 (2005).

156. Zhang, C., and Bennett, G.N., Biodegradation of xenobiotics by anaerobic bacteria, Appl. Microbiol. Biotechnol., 67, 600-618 (2005).

155. Cox, S.J., Shalel Levanon, S.S., Bennett, G.N., and San, K.-Y., Genetic network assisted metabolic flux analysis, Metabolic Eng. Sep-Nov;7(5-6):445-56 (2005).

154. Lin, H., Bennett, G.N., and San, K.-Y., Genetic reconstruction of the aerobic central metabolism in Escherichia coli for the absolute aerobic production of succinate, Biotech. & Bioeng., 89, 148-156 (2005).

153. Lin, H., Bennett, G.N., and San, K.-Y., Metabolic engineering of aerobic succinate production systems in Escherichia coli to improve process productivity and achieve the maximum theoretical succinate yield, Metabolic Eng., 7, 116-127 (2005).

152. Sanchez, A.M., Bennett, G.N., and San, K.-Y., Efficient succinate acid production from glucose through overexpression of pyruvate carboxylase in an Escherichia coli alcohol dehydrogenase and lactate dehydrogenase mutant, Biotechnol. Prog., 21, 358-365 (2005).

151. Zhao, Y., Tomas, C.A., Rudolph, F.B., Papoutsakis, E.T., and Bennett, G.N., Intracellular butyryl phosphate and acetyl phosphate concentrations in Clostridium acetobutylicum and their implications for solvent formation, Appl. Environ. Microbiol., 71, 530-537 (2005).

150. Shalel-Levanon.S., San, K.-Y., and Bennett, G.N., Effect of oxygen on the E. coli ArcA and FNR regulation systems and metabolic responses, Biotechnology and Bioengineering, 89, 556-564 (2005).

149. Ahmad, F., Hughes, J.B., and Bennett, G.N. Biodegradation of hazardous materials by Clostridia, Handbook on Clostridia (Durre, P., ed), Chapter 38, pp. 831-854, CRC Press, Boca Raton, FL (2005).

148. Ali, M.K., Rudolph, F.B., and Bennett, G.N., Characterization of thermostable Xyn10A enzyme from mesophilic Clostridium acetobutylicum strain ATCC 824, J. Ind. Microbiol. Biotechnol., 32, 12-18 (2004).

147. Lin, H., Vadali, R.V., Bennett, G.N., and San, K.-Y., Increasing the acetyl-CoA pool in the presence of overexpressed phosphoenolpyruvate carboxylase or pyruvate carboxylase enhances succinate production in Escherichia coli, Biotechnol. Prog., 20, 1599-1604 (2004).

146. Berrios-Rivera, S.J., Sánchez, A.M., Bennett, G.N., and San, K.-Y., Effect of different levels of NADH availability on metabolite distribution in Escherichia coli fermentation in minimal and complex media, Appl. Microbiol. Biotechnol., 65, 426-432 (2004).

145. Ali, M.K., Rudolph, F.B., and Bennett, G.N., Thermostable xylanase10B from Clostridium acetobutylicum ATCC824, J. Ind. Microbiol. Biotechnol. 31, 229-234 (2004). E pub June 8 (2004).

144. Vadali, R.V., Bennett, G.N., and San, K.-Y., Applicability of CoA/acetyl-CoA manipulation system to enhance isoamyl acetate production in Escherichia coli, Metabolic Eng., 6, 294-299 (2004).

143. Vadali, R.V., Bennett, G.N., and San, K.-Y., Enhanced isoamyl acetate production upon manipulation of the acetyl-CoA node in Escherichia coli, Biotechnol. Prog., 20, 692-697 (2004).

142. Vadali, R.V., Bennett, G.N., and San, K.-Y., Cofactor engineering of intracellular CoA/acetyl-CoA and its effect on metabolic flux redistribution in Escherichia coli, Metabolic Eng., 6, 133-139 (2004).

141. Vadali, R.V., Horton, C.E., Rudolph, F.B., Bennett, G.N., and San, K.Y., Production of isoamyl acetate in ackA-pta and/or ldh mutants of Escherichia coli with overexpression of yeast ATF2, Appl. Microbiol. Biotechnol., 63, 698-704 (2004).

140. San, K.-Y., and Bennett, G.N., Expression systems for DNA processes, in Encyclopedia of Molecular Cell Biology and Molecular Medicine: Vol. 4 (R.A. Meyers, ed), pp. 355-377, Wiley-VCH (2004).

139. Horton, C.E., Huang, K.-X., Bennett, G.N., and Rudolph, F.B., Heterologous expression of the Saccharomyces cerevisiae alcohol acetyltransferase genes in Clostridium acetobutylicum and Escherichia coli for the production of isoamyl acetate, J. Ind. Microbiol. Biotechnol., 30, 427-432 (2003).

138. Scotcher, M., Huang, K.-X., Harrison, M.L., Rudolph, F.B., and Bennett, G.N., Sequences affecting the regulation of solvent production in Clostridium acetobutylicum, J. Ind. Microbiol. Biotechnol., 30, 414-420 (2003).

137. Watrous, M.M., Clark, S., Kutty, R., Huang, S., Rudolph, F.B., Hughes, J.B., and Bennett, G.N., 2,4,6-Trinitrotoluene reduction by an Fe-only hydrogenase in Clostridium acetobutylicum, Applied and Environmental Microbiology, 69, 1542-1547 (2003).

136. Zhao, Y., Hindorff, L.A., Chuang, A., Monroe-Augustus, M., Lyristis, M., Harrison, M., Rudolph, F.B., and Bennett, G.N., Expression of a cloned cyclopropane fatty acid synthase gene reduces solvent formation in Clostridium acetobutylicum ATCC 824, Applied and Environmental Microbiology, 69, 2831-2841 (2003).

135. Padda, R.S., Wang, C., Hughes, J.B., Kutty, R., and Bennett, G.N., Mutagenicity of nitroaromatic compounds, Environ, Toxico. Chem. 22, 2293-2297 (2003).

134. Wang, C., Lyon, D.Y., Hughes, J.B.,, and Bennett, G.N., Role of hydroxylamine intermediates in the phytotransformation of 2,4,6-trinitrotoluene in Myriophyllum aquaticum, Environ. Sci. Technol. 37, 3595-3600 (2003).

133. Berrios-Rivera, S.J., San, K.-Y., and Bennett, G.N. The effect of carbon sources and lactate dehydrogenase deletion on 1,2-propanediol production in E. coli, J. Ind. Microbiol. Biotechnol., 30, 34-40 (2003).

132. Kim, H.-Y., Bennett, G.N., and Song, H.-G., Degradation of 2,4,6-trinitrotoluene by Enterobacter sp. strain C1 isolated from activated sludge, Biotechnology Letters 24, 2023-2028 (2002).

131. Oh, Y., Osato, M., Han, X., Bennett, G., and Hong, W.K., Folk yoghurt kills Helicobacter pylori, J. Appl. Microbiol. 93, 1083-1088 (2002).

130. Berrios-Rivera, S.J., San, K.-Y., and Bennett, G.N., The effect of NAPRTase overexpression on the total levels of NAD, NADH/NAD+ ratio, and the distribution of metabolites in Escherichia coli, Metabolic Eng., 4, 238-247 (2002).

129. Berrios-Rivera, S.J., Bennett, G.N., and San, K.-Y., The effect of increasing NADH availability on the redistribution of metabolic fluxes in Escherichia coli chemostat cultures, Metabolic Eng., 4, 230-237 (2002).

128. Berrios-Rivera, S.J., Bennett, G.N., and San, K.-Y., Metabolic engineering of Escherichia coli: Increase of NADH availability by overexpressing an NAD+-dependent formate dehydrogenase, Metabolic Eng., 4, 217-229 (2002).

127. San, K.-Y., Bennett, G.N., Berrios-Rivera, S.J., Vadali, R.V., Yang, Y.-T., Horton, E., Rudolph, F.B., Sariyar, B., and Blackwood, K., Metabolic engineering through cofactor manipulation and its effects on metabolic flux redistribution in Escherichia coli, Metabolic Eng., 4, 182-192 (2002).

126. Wardwell, S.A., Yang, Y.T., Chang, H.Y., San, K.Y., Rudolph, F.B., and Bennett, G.N., Expression of the Klebsiella pneumoniae CG21 acetoin reductase gene in Clostridium acetobutylicum ATCC 824, J. Ind. Microbiol. Biotechnol., 27, 220-227 (2001).

125. Nolling, J., Breton, G., Omelchenko, M.V., Makarova, K.S., Zeng, Q., Gibson, R., Lee, H.M., Dubois, J., Qiu, D., Hitti, J., Wolf, Y.I., Tatusov, R.L., Sabathe, F., Doucette-Stamm, L., Soucaille, P., Daly, M.J., Bennett, G.N., Koonin, E.V., and Smith, D.R., Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum, J. Bacteriol., 183, 4823-1438 (2001).

124. Tummala, S.B., Tomas, C., Harris, L.M., Welker, N.E., Rudolph, F.B., Bennett, G.N., and Papoutsakis, E.T., Genetic tools for solventogenic Clostridia, In Clostridia - Biotechnology and Medical Applications (H. Bahl, P. Dürre, eds.), pp. 105-123, Wiley-VCH, Weinheim, Germany (2001).

123. Bennett, G.N., and San, K-Y., Microbial formation, biotechnological production and applications of 1,2-propanediol, Appl. Microbiol. Biotechnol., 55, 1-9 (2001).

122. Yang, Y.-T., Bennett, G.N., and San, K.-Y., The effects of feed and intracellular pyruvate levels on the redistribution of metabolic fluxes in Escherichia coli , Metabolic Engineering 3, 115-123 (2001).

121. Loke, H.-K., Bennett, G.N., and Lindahl, P.A., Active acetyl-CoA synthase from Clostridium thermoaceticum obtained by cloning and heterologous expression of acsAB in Escherichia coli, Proc. National Acad. Sci. USA, 97, 12530-12535 (2000).

120. Yang, Y.-T., Peredelchuk, M., Bennett, G.N., and San, K.-Y., Effect of variation of Klebsiella pneumoniae acetolactate synthase expression on metabolic flux redistribution in Escherichia coli, Biotechnology & Bioengineering, 20, 150-159 (2000).

119. Berríos-Rivera, S.J., Yang, Y.-T., Bennett, G.N., and San, K.-Y., Effect of glucose analog supplementation on metabolic flux distribution in anaerobic chemostat cultures of Escherichia coli, J. Metabolic Engineering, 2, 149-154 (2000).

118. Huang, K.-x, Huang, S., Rudolph, F.B., and Bennett, G.N., Identification and characterization of a second butyrate kinase from Clostridium acetobutylicum ATCC 824, J. Mol.Microbiol. Biotechnol. 2, 33-38 (2000).

117. Padda, R.S., Wang, C.Y., Hughes, J.B., and Bennett, G.N., Mutagenicity of trinitotoluene and its metabolites formed during anaerobic degradation by Clostridium acetobutylicum ATCC 824, Environmental Toxicology and Chemistry. 19, 2871-2875 (2000).

116. Tyurin, M., Padda, R., Huang, K., Wardwell, S., Caprette, D., and Bennett, G.N., Electrotransformation of Clostridium acetobutylicum ATCC 824 using high-voltage radio frequency modulated square pulses, J. Appl. Microbiol. 88, 220-227 (2000).

115. Huang, S., Lindahl, P.A., Wang, C., Bennett, G.N., Rudolph, F.B., and Hughes, J.B., 2,4,6-Trinitrotoluene reduction by carbon monoxide dehydrogenase from Clostridium thermoaceticum, Appl. Environ. Microbiol. 66, 1474-1478 (2000).

114. Lyristis, M., Boynton, Z.L., Petersen, D., Kan, Z., Bennett, G.N., and Rudolph, F.B., Cloning, sequencing and characterization of the gene encoding flagellin, flaC, and the post-translational modification of the flagellin, FlaC, from Clostridium acetobutylicum ATCC824, Anaerobe 6, 69-79 (2000).

113. Yang, Y.-T., San, K.-Y., and Bennett, G.N., Redistribution of metabolic fluxes in Escherichia coli with fermentative lactate dehydrogenase overexpression and deletion, Metabolic Eng. 1, 141-152 (1999).

112. Yang, Y.-T., Bennett, G.N., and San, K.-Y., Effect of inactivation of nuo and ackA-pta on redistribution of metabolic fluxes in Escherichia coli, Biotech. Bioengineering 65, 291-297 (1999).

111. Huang, K-x., Scott, A.I., and Bennett, G.N., Overexpression, purification and characterization of the thermostable mevalonate kinase from Methanococcus jannaschii, Protein Expression and Purification 17, 33-40 (1999).

110. Papoutsakis, E.T., and Bennett, G.N., Molecular Regulation and Metabolic engineering of Solvent Production by Clostridium acetobutylicum, Chapter 11 in Metabolic Engineering (S.Y. Lee and E.T. Papoutsakis, eds.), Marcel Dekker, pp. 253-279 (1999).

109. Aristidou, A.A., San, K.-Y., and Bennett, G.N., Improvement of biomass yield and recombinant gene expression in Escherichia coli by using fructose as the primary carbon source, Biotech. Prog. 15, 140-145 (1999).

108. Huang, K.-x., Rudolph, F.B., and Bennett, G.N., Characterization of methylglyoxal synthase from Clostridium acetobutylicum ATCC 824 and its use in the formation of 1,2-propanediol, Appl. Environ. Microbiol. 65, 3244-3247 (1999).

107. Yang, Y.-T., San, K.-Y., and Bennett, G.N., Metabolic flux analysis of E. coli deficient in the acetate production pathway and expressing the B. subtilis acetolactate synthase, Metabolic Eng. 1, 26-34 (1999).

106. Aristidou, A.A., San, K.-Y., and Bennett, G.N., Metabolic flux analysis of E. coli expressing the B. subtilis ALS in batch and continuous culture, Biotech. Bioeng. 63, 737-749 (1999).

105. Nair, R.V., Green, E.M., Watson, D.E., Bennett, G.N., and Papoutsakis, E.T., Regulation of the Sol locus genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 by a putative transcriptional repressor, J. Bacteriol., 181, 319-330 (1999).

104. Yang, Y.-T., Bennett, G.N., and San, K.-Y., Genetic and metabolic engineering, Electronic Journal of Biotechnology, vol. 1, no. 3 (Dec. 15, 1998).

103. Hughes, J.B., Wang, C., Yesland, K., Richardson, A., Bhadra, R., Bennett, G., and Rudolph, F., Bamberger rearrangement during TNT-metabolism by Clostridium acetobutylicum, Environ. Sci. & Tech., 32, 494-500 (1998).

102. Hughes, J.B., Wang, C., Bhadra, R., Richardson, A., Bennett, G., and Rudolph, F., Reduction of 2,4,6-trinitrotoluene by Clostridium acetobutylicum through hydroxylamino-nitrotoluene intermediates, Environ. Tech. & Chem., 17, 343-348 (1998).

101. Belouski, E., Watson, D.E., and Bennett, G.N., Cloning, sequence and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli, Current Microbiol., 37, 17-22 (1998).

100. Belouski, E., Gui, L., Rudolph, F.B., and Bennett, G.N., Complementation of an Escherichia coli polypeptide deformylase mutant with a gene from Clostridium acetobutylicum ATCC 824, Current Microbiol., 36, 248-249 (1998).

99. Green, E.M., and Bennett, G.N., Genetic manipulation of acid and solvent formation in Clostridium acetobutylicum ATCC 824, Biotechnology & Bioengineering, 58, 215-221 (1998).

98. Watson, D.E., and Bennett, G.N., Cloning and assembly of PCR products using modified primers and DNA repair enzymes, Biotechniques 23, 858-864 (1997).

97. Peredelchuk, M.Y., and Bennett, G.N., A method for construction of E. coli strains with multiple DNA insertions in the chromosome, Gene, 187, 231-238 (1997).

96. Lin, J., Baik, H.S., Bennett, G.N., and Foster, J.W., Mechanisms of acid resistance and survival in enterohemorrhagic and enterotoxigenic Escherichia coli, Applied & Environ.Microbiol., 62, 3094-3100 (1996).

95. Boynton, Z.L., Bennett, G.N., and Rudolph, F.B., Cloning, sequencing and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824, Applied & Environ. Microbiol., 62, 2758-2766 (1996).

94. Fedorova, N.D., Peredelchuk, M.Y., Kirpichnikov, M.P., and Bennett, G.N., Escherichia coli strains for thermoinducible T7 RNA polymerase-driven expression, Gene, 177, 267-268 (1996).

93. Stim-Herndon, K.P., Nair, R., Papoutsakis, E.T., and Bennett, G.N., Analysis of degenerate variants of Clostridium acetobutylicum ATCC 824, Anaerobe, 2, 11-18 (1996).

92. Wong, J., and Bennett, G.N., Recombination-induced variants of Clostridium acetobutylicum ATCC 824 with increased solvent production, Current Microbiology, 32, 349-356 (1996).

91. Wong, J., and Bennett, G.N., The effect of novobiocin on solvent production by Clostridium acetobutylicum, J. Industrial Microbiol., 16, 354-359 (1996).

90. Stim-Herndon, K.P., Flores, T.M., and Bennett, G.N., Molecular characterization of adiY, a regulatory gene which affects expression of the biodegradative acid-induced arginine decarboxylase gene (adiA) of Escherichia coli, Microbiology, 142, 1311-1320 (1996).

89. Chou, C.-H., Bennett, G.N., and San, K.-Y., Genetic manipulation of stationary-phase genes to enhance recombinant protein production in Escherichia coli, Biotechnol. & Bioeng., 50, 636-642 (1996).

88. Boynton, Z.L., Bennett, G.N., and Rudolph, F.B., Cloning, sequencing and expression of clustered genes encoding b-hydroxybutyryl-Coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824, J. Bacteriology, 178, 3015-3024 (1996).

87. Green, E.M., Boynton, Z.L., Harris, L.M., Rudolph, F.B., Papoutsakis, E.T., and Bennett, G.N., Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824, Microbiology, 142, 2079-2086 (1996).

86. Bennett, G.N., and Rudolph, F.B., The central metabolic pathway from acetyl-CoA to butyryl-CoA in Clostridium acetobutylicum, FEMS Microbiol. Reviews, 17, 241-249 (1995).

85. Chou, C.H., Aristidou, A.A., Meng, S.Y., Bennett, G.N., and San, K.-Y., Characterization of a pH inducible promoter system for high level expression of recombinant proteins in Escherichia coli, Biotechnol. & Bioeng., 47, 186-192 (1995).

84. San, K.-Y., and Bennett, G.N., Expression systems for DNA processes, in Encyclopedia of Molecular Biology: Fundamentals and Applications (R.A. Meyers, ed.), VCH Publishers, Weinheim, pp. 271-278 (1995).

83. Aristidou, A.A., San, K.-Y., and Bennett, G.N., Metabolic engineering of Escherichia coli to enhance recombinant protein production through acetate reduction, Biotechnol. Prog. 11, 475-478 (1995).

82. Stim-Herndon, K.P., Petersen, D.J., and Bennett, G.N., Characterization of an acetyl CoA- acetyltransferase (thiolase) gene from Clostridium acetobutylicum ATCC 824, Gene, 154, 81-85 (1995).

81. Wong, J., Sass, C., and Bennett, G.N., Sequence and arrangement of genes encoding sigma factors in Clostridium acetobutylicum ATCC 824, Gene, 153, 89-92 (1995).

80. Shi, X., and Bennett, G. N., Effects of multicopy leuO on the expression of the acid-inducible lysine decarboxylase gene in Escherichia coli, J. Bact., 177, 810-814 (1995).

79. San, K.-Y., and Bennett, G.N., Expression systems for DNA processes, in Molecular Biology and Biotechnology: A Comprehensive Desk Reference (R.A. Meyers, ed.), VCH Publishers, New York, pp. 306-310 (1995).

78. Chou, C.-H., Bennett, G.N., and San, K.-Y., Effect of modulated glucose uptake on high-level recombinant protein production in a dense Escherichia coli culture, Biotechnol. Prog., 10, 644-647 (1994).

77. Shi, X., and Bennett, G.N., Effects of rpoA and cysB mutations on acid induction of biodegradative arginine decarboxylase in Escherichia coli, J. Bact. 176, 7017-7023 (1994).

76. Shi, X., and Bennett, G.N., Plasmids bearing hfq and the hns-like gene stpA complement hns mutants in modulating arginine decarboxylase gene expression in Escherichia coli, J. Bacteriol. 176, 6769-6775 (1994).

75. Chou, C.-H., Bennett, G.N., and San, K.-Y., Effect of modified glucose uptake using genetic engineering technique, on high-level recombinant protein production in Escherichia coli dense cultures, Biotechnol. & Bioeng. 44, 952-960 (1994).

74. Aristidou, A.A., San, K.-Y., and Bennett, G.N., Modification of central metabolic pathway in Escherichia coli to reduce acetate accumulation by heterologous expression of the Bacillus subtilis acetolactate synthase gene, Biotechnol. & Bioeng. 44, 944-951 (1994).

73. San, K.-Y., Bennett, G.N., Aristidou, A.A., and Chou, C.-H., Strategies in high-level expression of recombinant protein in Escherichia coli, Ann. N.Y. Acad. Sci., 721, 257-267 (1994).

72. Mermelstein, L.D., Welker, N.E., Petersen, D.J., Bennett, G.N., and Papoutsakis, E.T., Genetic and metabolic engineering of Clostridium acetobutylicum ATCC 824, Ann. N.Y. Acad. Sci., 721, 54-68 (1994).

71. San, K.-Y., Bennett, G.N., Chou, C.-H., and Aristidou, A.A., An optimization study of a pH-inducible promoter system for high-level recombinant protein production in Escherichia coli, Ann. N.Y. Acad. Sci., 721, 268-276 (1994).

70. Nair, R.V., Bennett, G.N., and Papoutsakis, E.T., Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824, J. Bacteriol., 176, 871-885 (1994).

69. Boynton, Z.L., Bennett, G.N., and Rudolph, F.B., Intracellular concentrations of coenzyme A and its derivatives from Clostridium acetobutylicum ATCC 824 and their roles in enzyme regulation, Applied Environ. Microbiol., 60, 39-44 (1994).

68. Mermelstein, L.D., Papoutsakis, E.T., Petersen, D.J., and Bennett, G.N., Metabolic engineering of Clostridium acetobutylicum ATCC 824 for increased solvent formation by enhancement of acetone formation enzyme activities using a synthetic acetone operon, Biotechnology & Bioengineering, 42, 1053-1060 (1993).

67. Walter, K.A., Nair, R.V., Cary, J.W., Bennett, G.N., and Papoutsakis, E.T., Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824, Gene, 134, 107-111 (1993).

66. Papoutsakis, E.T., and Bennett, G.N., Cloning, structure and expression of acid and solvent path­way genes of Clostridium acetobutylicum, in The Clostridia and Biotechnology, Chap. 8 (D.R. Woods, ed.), Butterworth-Heinemann Pubs., Stoneham, Massachusetts, pp. 157-199 (1993).

65. Papoutsakis, E.T., Mermelstein, L.D., Welker, N.E., Petersen, D.J., and Bennett, G.N., Genetic and metabolic engineering of Clostridium acetobutylicum, Proceedings of NIH Conference: "Research Opportunities in Biomolecular Engineering: The Interface between Chemical Engineering and Biology," pp. 77-83, NIH/NIGMS, Washington, D.C. (1993).

64. Mermelstein, L.D., Bennett, G.N., and Papoutsakis, E.T., Amplification of homologous fermenta­tive genes in Clostridium acetobutylicum ATCC 824, in Proceedings from the Third U.S./Japan Conference on Biotechnology (R. Tanner, ed.), Springer-Verlag, pp. 81-88 (1993).

63. Stim, K., and Bennett, G.N., Nucleotide sequence of the adi gene, which encodes the biodegradative acid-induced arginine decarboxylase of Escherichia coli, J. Bact., 175, 1221-1234 (1993).

62. Shi, X, Waasdorp, B.C., and Bennett, G.N., Modulation of acid-induced amino acid decarboxylase gene expression by hns in Escherichia coli, J. Bact., 175, 1182-1186 (1993).

61. Sass, C., Walter, J., and Bennett, G.N., Isolation of mutants of Clostridium acetobutylicum ATCC 824 deficient in protease activity, Current Microbiology, 26, 151-154 (1993).

60. Petersen, D.J., Cary, J.W., Vanderleyden, J., and Bennett, G.N., Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC 824, Gene, 123, 93-97 (1993).

59. Bennett, G.N., and Petersen, D.J., Cloning and expression of Clostridium acetobutylicum genes in­volved in solvent production, in Genetics and Molecular Biology of Anaerobes, Chap. 22 (M. Sebald, ed.), Springer Verlag, New York, pp. 317-343 (1993).

58. Walter, K.A., Bennett, G.N., and Papoutsakis, E.T., Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes, J. Bacteriol., 174, 7149-7158 (1992).

57. Lee, S.Y., Mermelstein, L.D., Bennett, G.N., and Papoutsakis, E.T., Vector construction, trans­formation, and gene amplification in Clostridium acetobutylicum ATCC 824, Biochem. Eng., VII, Ann. New York Acad. Sci., 665, 39-51 (1992).

56. Hassani, M., Pincus, D.H., Bennett, G.N., and Hirshfield, I.N., Temperature-dependent induction on an acid-inducible stimulon of Escherichia coli in broth, App. Environ. Microbiol., 58, 2704-2707 (1992).

55. Welch, R.W., Clark, S.W., Bennett, G.N., and Rudolph, F.B., Effects of rifampicin and chlo­ramphenical on product and enzyme levels of the acid- and solvent-producing pathways of Clostridium acetobutylicum (ATCC 824), Enzyme Microbiol. Technol., 14, 277-283 (1992).

54. Tolentino, G.J., Meng, S.Y., Bennett, G.N., and San, K.-Y., A pH-regulated promoter for the ex­pression of recombinant proteins in Escherichia coli, Biotechnology Letters, 14, 157-162 (1992).

53. Lee, S.Y., Bennett, G.N., and Papoutsakis, E.T., Construction of Escherichia coli-Clostridium acetobutylicum shuttle vectors and transformation of Clostridium acetobutylicum strains, Biotechnology Letters, 14, 427-432 (1992).

52. Mermelstein, L.D., Welker, N.E., Bennett, G.N., and Papoutsakis, E.T., Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824, Bio/Technology, 10, 190-195 (1992).

51. Meng, S.-Y., and Bennett, G.N., Regulation of the Escherichia coli cad operon: Location of a site re­quired for acid induction, J. Bacteriol., 174, 2670-2678 (1992).

50. Meng, S.-Y., and Bennett, G.N., Nucleotide sequence of the Escherichia coli cad operon: A system for detoxification of low extracellular pH, J. Bacteriol., 174, 2659-2669 (1992).

49. Petersen, D.J., Welch, R.W., Walter, K.A., Mermelstein, L.D., Papoutsakis, E.T., Rudolph, F.B., and Bennett, G.N., Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium acetobutylicum, Ann. N.Y. Acad. Sci., 646, 94-98 (1991).

48. Petersen, D.J., and Bennett, G.N., Enzymatic characterization of a nonmotile, nonsolventogenic Clostridium acetobutylicum ATCC 824 mutant, Current Microbiol., 23, 253-258 (1991).

47. Petersen, D.J., and Bennett, G.N., Cloning of the Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase (Thiolase; EC 2.3.1.9) gene, Appl. Environ. Microbiol., 57, 2735-2741 (1991).

46. Hassani, M., Saluta, M.V., Bennett, G.N., and Hirshfield, I.N., Partial characterization of a lysU mutant of Escherichia coli K-12, J. Bacteriol., 173, 1965-1970 (1991).

45. Vermersch, P.S., and Bennett, G.N., Stability of mutant Type II dihydrofolate reductase proteins in suppressor strains, J. Biotechnology, 19, 49-66 (1991).

44. Petersen, D.J., Welch, R.W., Rudolph, F.B., and Bennett, G.N., Molecular cloning of an alcohol (butanol) dehydrogenase gene cluster from Clostridium acetobutylicum ATCC 824, J. Bacteriol., 173, 1831-1834 (1991).

43. Wang-Bennett, L.T., Liebl, D.J., and Bennett, G.N., Targeted neuronal lesion induced by photo­sensitizing dyes, Brain Research, 534, 122-128 (1990).

42. Petersen, D.J., and Bennett, G.N., Purification of acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824 and cloning of the acetoacetate decarboxylase gene in Escherichia coli, App. Environ. Microb., 56, 3491-3498 (1990).

41. Cary, J.W., Petersen, D.J., Papoutsakis, E.T., and Bennett, G.N., Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-Coenzyme A:acetate/butyrate:Coenzyme A-transferase in Escherichia coli, App. Environ. Microb., 56, 1576-1583 (1990).

40. Brito, R.M.M., Reddick, R., Bennett, G.N., Rudolph, F.B., and Rosevear, P.R., Characterization and stereo­chemistry of cofactor oxidation by a type II dihydrofolate reductase, Biochemistry, 29, 9825-9831 (1990).

39. Cary, J.W., Petersen, D.J., Bennett, G.N., and Papoutsakis, E.T., Methods for cloning key pri­mary metabolic enzymes and ancillary proteins associated with the acetone-butanol fermentation of Clostridium acetobutylicum, Ann. N.Y. Acad. Sci., 589, 67-81 (1990).

38. Clark, S.W., Bennett, G.N., and Rudolph, F.B., Isolation and characteriza­tion of mutants of Clostridium acetobutylicum ATCC 824 deficient in acetoacetyl-Coenzyme A:acetate/butyrate: Coenzyme A transferase (EC 2.8.3.9) and in other solvent pathway enzymes, App. Environ. Microb., 55, 970-976 (1989).

37. Auger, E.A., Redding, K.E., Plumb, T., Childs, L.C., Meng, S.-Y., and Bennett, G.N., Construc­tion of lac fusions to the inducible arginine- and lysine decarboxylase genes of Escherichia coli K12, Molecular Microbiol., 3, 609-620 (1989).

36. Auger, E.A., and Bennett, G.N., Regulation of lysine decarboxylase activity in Escherichia coli K-12, Arch. Microbiol., 15, 466-468 (1989).

35. Cary, J.W., Petersen, D.J., Papoutsakis, E.T., and Bennett, G.N., Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli, J. Bacteriol., 170, 4613-4618 (1988).

34. Vermersch, P.S., and Bennett, G.N., Synthesis and expression of a gene for a mini Type II dihydro­fo­late reductase, DNA, 7, 243-251 (1988).

33. Vermersch, P.S., and Bennett, G.N., The use of a selectable FokI cassette in DNA replacement muta­genesis of the R388 dihydrofolate reductase gene, Gene, 54, 229-238 (1987).

32. Gayle, R.B., III, Auger, E.A., Gough, G.R., Gilham, P.T., and Bennett, G.N., Formation of MboII vectors and cassettes using asymmetric MboII linkers, Gene, 54, 221-228 (1987).

31. Auger, E.A., and Bennett, G.N., Temperature optimization of in vivo expression from the E. coli trp and trp::lac promoters, Biotechnology Letters, 9 157-162 (1987).

30. Herrin, G.L., Jr., and Bennett, G.N., The effects of nalidixic acid on expres­sion from related E. coli promoters, Biochem. Biophys. Res. Commun., 135, 411-418 (1986).

29. Vermersch, P.S., Klass, M.R., and Bennett, G.N., Use of bacterial DHFR-II fusion proteins to elicit specific antibodies, Gene, 41, 289-297 (1986).

28. Gayle, R.B., III, Vermersch, P.S., and Bennett, G.N., Construction and charac­teri­zation of pBR322-derived plasmids with deletions of the RNA I region, Gene, 41, 281-288 (1986).

27. Russell, D.R., Miller, P.D., and Bennett, G.N., In vitro characterization of hy­brid promoters and al­tered tryptophan operon promoters, Biochemistry, 24, 1410-1417 (1985).

26. Manly, S.P., Bennett, G.N., and Matthews, K.S., Enzymatic digestion of operator DNA in the pres­ence of the lac repressor tryptic core, J. Mol. Biol., 179, 335-350 (1984).

25. Herrin, G.L., Jr., and Bennett, G.N., Role of DNA regions flanking the tryptophan pro­moter of Escherichia coli. II. Insertion of lac operator fragments, Gene, 32, 349-356 (1984).

24. Russell, D.R., Auger, E.A., Vermersch, P.S., and Bennett, G.N., Role of DNA regions flanking the tryptophan promoter of Escherichia coli. I. Insertion of synthetic oligonucleotides, Gene, 32, 337-348 (1984).

23. Manly, S.P., Bennett, G.N., and Matthews, K.S., Perturbation of lac operator DNA modification by tryptic core protein from lactose repressor, Proceedings of the National Academy of Sciences U.S.A., 80, 6219-6223 (1983).

22. Russell, D.R., and Bennett, G.N., Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spac­ing, Gene, 20, 231-243 (1982).

21. Bennett, G.N., Formation of alkali labile linkages in DNA by hedamycin and use of hedamycin as a probe of protein-DNA complexes, Nucleic Acids Research, 10, 4581-4594 (1982).

20. Herrin, G.L., Jr., Russell, D.R., and Bennett, G.N., A stable derivative of pBR322 conferring in­creased tetracycline resistance and increased sensitivity to fusaric acid, Plasmid, 7, 290-293 (1982).

19. Russell, D.R., and Bennett, G.N., Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator, Gene, 17, 9-18 (1982).

18. Russell, D.R., and Bennett, G.N., Characterization of the b-lactamase promoter of pBR322, Nucleic Acids Research, 9, 2517-2533 (1981).

17. Sumner, W., II, and Bennett, G.N., Anthramycin inhibition of restriction endonu­cle­ase cleavage and its use as a reversible blocking agent in DNA construc­tions, Nucleic Acids Research, 9, 2105-2119 (1981).

16. Oppenheim, D.S., Bennett, G.N., and Yanofsky, C., Escherichia coli RNA polymer­ase and trp re­pressor interaction with the promoter-operator region of the trypto­phan operon of Salmonella typhimurium, J. Mol. Biol., 144, 133-142 (1980).

15. Nichols, B.P., Miozzari, G.F., van Cleemput, M., Bennett, G.N., and Yanofsky, C., Nucleotide se­quences of the trp G regions of Escherichia coli, Shigella dysenteriae, Salmonella typhimurium and Serratia marcescens, J. Mol. Biol., 142, 503-517 (1980).

14.  Franklin, N.C., and Bennett, G.N., The N-protein of bacteriophage lambda, defined by its DNA se­quence, is highly basic, Gene, 8, 107-119 (1979).

13. Stauffer, G.V., Zurawski, G., and Bennett, G.N., In vivo cloning of DNA regions carrying muta­tions linked to selectable genes: Application to mutations in the regulatory region of the Escherichia coli tryptophan operon, Plasmid, 2, 498-502 (1979).

12. Lee, F., Bertrand, K., Bennett, G., and Yanofsky, C., Comparison of the nucleotide sequences of the initial transcribed regions of the tryptophan operons of Escher­i­chia coli and Salmonella typhimurium, J. Mol. Biol., 121, 193-217 (1978).

11. Bennett, G.N., and Yanofsky, C., Sequence analysis of operator constitutive mu­tants of the trypto­phan operon of Escherichia coli, J. Mol. Biol., 121, 179-192 (1978).

10. Brown, K.D., Bennett, G.N., Lee, F., Schweingruber, M.E., and Yanofsky, C., RNA polymerase interaction at the promoter-operator region of the tryptophan operon of Escherichia coli and Salmonella typhimurium, J. Mol. Biol., 121, 153-177 (1978).

9. Bennett, G.N., Brown, K.D., and Yanofsky, C., Nucleotide sequence of the promoter-operator re­gion of the tryptophan operon of Salmonella typhimurium, J. Mol. Biol., 121, 139-152 (1978).

8. Bennett, G.N., Schweingruber, M.E., Brown, K.D., Squires, C., and Yanofsky, D., Nucleotide se­quences of the promoter-operator region of the tryptophan operon of Escher­ichia coli, J. Mol. Biol. 121, 113-137 (1978).

7. Hawley, D.M., Sninsky, J.J., Bennett, G.N., and Gilham, P.T., Activity of poly­nucleotide phos­phorylase with nucleoside diphosphates containing sugar ring modi­fications, Biochemistry, 17, 2082-2086 (1978).

6. Bennett, G.N., Gough, G.R., and Gilham, P.T., Guanosine tetraphosphate and its analogs. Chemical synthesis of guanosine 3',5'-di(pyrophosphate), deoxyguano­sine 3',5'-di(pyrophosphate), guano­sine 2',5'-bis(methylenediphosphonate), and guano­sine 3',5'-bis(methylenediphosphonate), Biochemistry, 15, 4623-4628 (1976).

5. Bennett, G.N., Schweingruber, M.E., Brown, K.D., Squires, C., and Yanofsky, C., Nucleotide se­quence of region preceding trp mRNA initiation site and its role in promoter and operator function, Proceedings of the National Academy of Scien­ces U.S.A., 73, 2351-2355 (1976).

4. Bennett, G.N., and Gilham, P.T., "Single-addition" substrates for the synthesis of specific oligori­bonucleotides with polynucleotide phosphorylase. Synthesis of 2'-O-(a-methoxylethyl)-nucleoside 5'-diphosphates, Biochemistry, 14, 3152-3158 (1975).

3. Bennett, G.N., The use of the methoxyethyl blocking group in the enzymatic syn­­the­sis of specific oligonucleotides and in the chemical synthesis of guanosine tetraphosphate, Ph.D. Thesis, Purdue University (1974).

2. Sninsky, J.J., Bennett, G.N., and Gilham, P.T., "Single-addition" and "trans­nu­cleo­tidation" reac­tions catalyzed by polynucleotide phosphorylase. Effect of enzy­matic removal of inorganic phos­phate during reaction, Nucleic Acids Re­search, l, 1665-1674 (1974).

1. Bennett, G.N., Mackey, J.K., Weibers, J.L., and Gilham, P.T., 2'-O-(a-metho­xylethyl)-nucleoside 5'-diphosphates as "single-addition" substrates in the syn­thesis of specific oligoribonucleotides with polynucleotide phosphorylase, Biochemistry, 12, 3956-3962 (1973).